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KMID : 1025520010430010035
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Journal of Animal Science and Technology
2001 Volume.43 No. 1 p.35 ~ p.44
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Genetic Diversity and Parentage Testing of Korean Cattle using VNTR Markers
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Abstract
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VNTR(Variable Number of tandem repeats) loci also known as hypervariable minisatellite loci are regions of DNA with highly polymorphisms in the number of tandemly repeated nucleotide sequences of around 25bp in length. This study was conducted to investigate allele frequencies and polymorphic information of BTGL1 VNTR locus and to check pedigree records by parentage testing and pedigree analysis of paternal half-sib families using VNTR markers in Korean cattle. The BTGL1 minisatellite locus was amplified by PCR and the DNA fragments were size-separated by non-denaturing polyacrylamide gels followed by silver staining. A total of 15 different alleles ranging in size from approximately 302 to 440bp were identified in the BTGL1 locus. The heterozygosity(H) and polymorphic information content(PIC) values were 0.877 and 0.828 in Korean cattle population, respectively. Mendelian segregation and codominant inheritance for the alleles of VNTR loci were confirmed in paternal half-sib families of Korean cattle. The allelic bands seen in the offsprings of each pedigree were always observed from either sire or dam. However, among the thirteen paternal half-sib families examined, the falsely recorded pedigrees were found in three families having non-paternal bands. Therefore, the relationships between parents and offspring in these three families can be excluded. The BTGL1 VNTR DNA polymorphisms could be used as genetic markers for routine parentage testing and pedigree analysis in Korean cattle.
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